Gingivitis, a common and mild form of gum disease, can progress to periodontitis, which is a more serious infection that damages the gums and sometimes even destroys the bone supporting the teeth. An international team of researchers and clinicians has charted the microbial ecology of the mouth at all stages of this progression in nearly 1,000 women in Malawi. This work is laying a foundation of knowledge that could lead to better oral health. The research is published in Applied and Environmental Microbiology, a journal of the American Society for Microbiology.
The investigators used high-throughput sequencing of the 16S ribosomal RNA (rRNA) gene to take the census of the oral microbiomes. Among much else, they found that a small number of species were associated with periodontitis but not gingivitis, including members of the genera Prevotella, Treponema and Selemonas. “Our findings confirm that periodontitis cannot be considered simply an advanced stage of gingivitis even when only considering supragingival plaque,” said first author Mr. Liam Shaw, a PhD student at University College London, United Kingdom.
Periodontitis is diagnosed by measuring the depth of the gum pockets. “But diagnosing periodontitis visually is impossible and it doesn’t usually give any symptoms until it has developed so far that teeth become mobile, which is very late for any treatment,” said co-author Dr. Ulla Harjunmaa, a dentist with a master’s degree in international Health, who is a PhD student at the Centre for Child Health Research, University of Tampere and Tampere University Hospital, Finland.
Diagnosis requires specially trained dental professionals and expensive equipment, which are seldom available in developing countries, said Dr. Harjunmaa. But among other things, in the future, this research may lead to an inexpensive and rapid point-of-care test, based on distinguishing the two conditions’ microbiomes. “The research made it possible to tell which differences in bacteria were linked specifically to the deepening of dental pockets – which causes loss of teeth – rather than just to bleeding gums,” explained Mr. Shaw.
“We’re not the first to do this sort of research into periodontal disease, but our study is the largest to be done in such a setting,” said Mr. Shaw. “There is a great need to find new solutions to prevent these problems everywhere, but especially in this kind of setting.” He added that oral infections are very common in Malawi, since there is little awareness about oral health and prevention, and little treatment available.
Some of the techniques used in the research may have wide applications, said Mr. Shaw. “Where diseases can have multiple severities and clinical features that are also found in non-disease (like bleeding gums in periodontitis), this kind of modeling will be increasingly important in microbiome research.”
Periodontal disease is a major public health problem associated with oral bacteria. In developed nations, approximately half of adults are thought to have moderate periodontal disease, with another 7 to 15 per cent having advanced disease, according to the Centres for Disease Control and Prevention. Additionally, periodontal disease is thought to be a risk factor for cardiovascular disease, diabetes and other chronic health conditions. Treating gum disease could reduce healthcare costs, as well as hospitalisations, according to a study published in the August 2014 American Journal of Preventive Medicine.
“The best way to prevent periodontal diseases is by proper toothbrushing twice a day combined with flossing once a day,” said Dr. Harjunmaa. For smokers, quitting is also critically important to prevention, she said.